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irdye 680rd donkey anti mouse secondary antibody  (LI-COR)


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    Structured Review

    LI-COR irdye 680rd donkey anti mouse secondary antibody
    Irdye 680rd Donkey Anti Mouse Secondary Antibody, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1320 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/irdye+680rd+donkey+anti+mouse+secondary+antibody/DONKEYaMOUSE+IRDye+680RD/pm42045653-265-38-44
    Average 99 stars, based on 1320 article reviews
    irdye 680rd donkey anti mouse secondary antibody - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Control:

    Article Title: Small-molecule binding-site discovery using silyl ether-enabled chemoproteomics.
    Article Snippet: After blocking, the membrane was incubated with one of the rabbit primary antibodies listed below at a ratio of 1:3,000 in 5% (w/v) milk in 1× TBS overnight at 4 °C, washed with 1× TBS for 10 min three times the following day and incubated with a secondary antibody, IRDye 800CW goat anti-rabbit secondary antibody (Li-Cor Biotechnology, 926-32211, #D50528-07), at a ratio of 1:5,000 in 5% (w/v) milk in 1× TBST (TBS with 0.1% Tween20) room temperature for 1 h. After secondary antibody incubation, the membrane was washed with 1× TBS for 10 min three times and imaged using a Bio-Rad ChemiDoc Imaging System to obtain western blot results. .. For loading control, the membrane underwent the similar western blot analysis as described above using mouse anti-β-actin antibody (Cell Signaling, #3700S, #21) or mouse GAPDH monoclonal antibody (Proteintech, #60004-1-Ig, #10080731) as a primary antibody at 1:3,000 dilution and IRDye 680RD donkey anti-mouse secondary antibody (Li-Cor Biotechnology, 926-68072, #D41217-05) as a secondary antibody at 1:5000 dilution. .. For transfection done in a six-well plate, plasmid (1.5 μg) and PEI MAX-Transfection Grade Linear Polyethylenimine Hydrochloride (MW 40,000) (Polysciences, 24765-1, 7.5 μl) were each diluted with OptiMEM (75 μl).

    Membrane:

    Article Title: Small-molecule binding-site discovery using silyl ether-enabled chemoproteomics.
    Article Snippet: After blocking, the membrane was incubated with one of the rabbit primary antibodies listed below at a ratio of 1:3,000 in 5% (w/v) milk in 1× TBS overnight at 4 °C, washed with 1× TBS for 10 min three times the following day and incubated with a secondary antibody, IRDye 800CW goat anti-rabbit secondary antibody (Li-Cor Biotechnology, 926-32211, #D50528-07), at a ratio of 1:5,000 in 5% (w/v) milk in 1× TBST (TBS with 0.1% Tween20) room temperature for 1 h. After secondary antibody incubation, the membrane was washed with 1× TBS for 10 min three times and imaged using a Bio-Rad ChemiDoc Imaging System to obtain western blot results. .. For loading control, the membrane underwent the similar western blot analysis as described above using mouse anti-β-actin antibody (Cell Signaling, #3700S, #21) or mouse GAPDH monoclonal antibody (Proteintech, #60004-1-Ig, #10080731) as a primary antibody at 1:3,000 dilution and IRDye 680RD donkey anti-mouse secondary antibody (Li-Cor Biotechnology, 926-68072, #D41217-05) as a secondary antibody at 1:5000 dilution. .. For transfection done in a six-well plate, plasmid (1.5 μg) and PEI MAX-Transfection Grade Linear Polyethylenimine Hydrochloride (MW 40,000) (Polysciences, 24765-1, 7.5 μl) were each diluted with OptiMEM (75 μl).

    Western Blot:

    Article Title: Small-molecule binding-site discovery using silyl ether-enabled chemoproteomics.
    Article Snippet: After blocking, the membrane was incubated with one of the rabbit primary antibodies listed below at a ratio of 1:3,000 in 5% (w/v) milk in 1× TBS overnight at 4 °C, washed with 1× TBS for 10 min three times the following day and incubated with a secondary antibody, IRDye 800CW goat anti-rabbit secondary antibody (Li-Cor Biotechnology, 926-32211, #D50528-07), at a ratio of 1:5,000 in 5% (w/v) milk in 1× TBST (TBS with 0.1% Tween20) room temperature for 1 h. After secondary antibody incubation, the membrane was washed with 1× TBS for 10 min three times and imaged using a Bio-Rad ChemiDoc Imaging System to obtain western blot results. .. For loading control, the membrane underwent the similar western blot analysis as described above using mouse anti-β-actin antibody (Cell Signaling, #3700S, #21) or mouse GAPDH monoclonal antibody (Proteintech, #60004-1-Ig, #10080731) as a primary antibody at 1:3,000 dilution and IRDye 680RD donkey anti-mouse secondary antibody (Li-Cor Biotechnology, 926-68072, #D41217-05) as a secondary antibody at 1:5000 dilution. .. For transfection done in a six-well plate, plasmid (1.5 μg) and PEI MAX-Transfection Grade Linear Polyethylenimine Hydrochloride (MW 40,000) (Polysciences, 24765-1, 7.5 μl) were each diluted with OptiMEM (75 μl).

    Blocking Assay:

    Article Title: 2-Deoxy-D-glucose inhibits lymphocytic choriomeningitis virus propagation by targeting glycoprotein N-glycosylation
    Article Snippet: Membranes were blocked with 5% nonfat dry milk in Tris-buffered saline with 0.1% Tween 20 (TBST) for 1 h and then probed with indicated primary antibodies diluted in Odyssey blocking buffer (Li-Cor) for 1 h at room temperature or overnight at 4 °C (dilutions: 1:500 for anti-GP2, 1:1,000 for anti-NP, 1:10,000 for anti-α-tubulin, 1:10,000 for anti-β-actin, and undiluted culture medium for anti-Z). .. Membranes were washed 5 × with TBST and then probed with peroxidase-conjugated goat anti-mouse or IRDye ® 680RD donkey anti-mouse secondary antibody diluted in Li-Cor blocking buffer (1:10,000) for 45 min at room temperature. .. Subsequently, the membranes were washed 5× with TBST, and immunoreactive proteins were visualized either by chemiluminescence or were scanned with an Odyssey CLx Imaging System (Li-Cor).

    other:

    Article Title: Effects of doxorubicin on autophagy in fibroblasts
    Article Snippet: IRDye ® 680RD donkey anti-mouse secondary antibody , LI-COR biotechnology , 926–68072 , 1:20,000.

    Article Title: Heat shock induces premature transcript termination and reconfigures the human transcriptome
    Article Snippet: IRDye 680RD Donkey anti-Mouse secondary antibody , LI-COR Biosciences , Cat# 926-68072 RRID: AB_10953628.

    Incubation:

    Article Title: Understanding the role of the hematopoietic niche in Huntington's disease's phenotypic expression: in vivo evidence using a parabiosis model.
    Article Snippet: To detect total HTT and mHTT within organs, 50 μg of proteins were partitioned on a 6% SDS-polyacrylamide gel for 3 h at 110 V and transferred overnight (O/N) at 20 V onto a low fluorescence polyvinylidene difluoride (PVDF) membrane (Immobilon-FL MerckMillipore #IPFL00010) in Towbin transfer buffer containing 0.01% SDS and 16% methanol. .. All membranes were blocked with 2.5% BSA in TBS and incubated O/N at 4 ◦C with primary antibody (polyglutamine, 1:1000, Merck-Millipore #MAB1574 and total HTT, 1:1000, MerckMillipore #MAB2166) in TBS containing 0.1% Tween-20 (TBST), followed by the IRDye 680RD donkey anti-mouse secondary antibody (1:20000, LI-COR Biotechnology, #925–68072) for 45 min at RT. .. Fluorescent signal was acquired and quantified using the Odyssey Imaging System (Odyssey; Li-Cor, Lincoln, NE).



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